Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Receptor-mediated control of regulatory volume decrease (RVD) and apoptotic volume decrease (AVD).

Y Okada1, E Maeno, T Shimizu

  • 1Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan. okada@nips.ac.jp

The Journal of Physiology
|April 3, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Normotonic cell shrinkage induces apoptosis under extracellular low Cl conditions in human lymphoid and epithelial cells.

Acta physiologica (Oxford, England)·2006
Same author

Volume-sensitive chloride channels involved in apoptotic volume decrease and cell death.

The Journal of membrane biology·2006
Same author

Cells die with increased cytosolic ATP during apoptosis: a bioluminescence study with intracellular luciferase.

Cell death and differentiation·2005
Same author

Apoptotic and necrotic blebs in epithelial cells display similar neck diameters but different kinase dependency.

Cell death and differentiation·2003
Same author

Apoptosis, cell volume regulation and volume-regulatory chloride channels.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2002
Same author

Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis.

Proceedings of the National Academy of Sciences of the United States of America·2000
Same journal

Improved subjective sleep quality in older adults by enhancing the GABAergic system in the sensorimotor cortex.

The Journal of physiology·2026
Same journal

Fasting-induced glucose intolerance is not impacted by the addition of daily exercise: A randomized crossover study.

The Journal of physiology·2026
Same journal

Diving exposure and pulmonary stress.

The Journal of physiology·2026
Same journal

Systems modelling of mitochondrial dynamics in different exercise regimes.

The Journal of physiology·2026
Same journal

Central leptin resistance precedes obesity and drives early endocrine dysfunction.

The Journal of physiology·2026
Same journal

Decoding the molecular memory of obesity using machine learning and microRNA dynamics.

The Journal of physiology·2026
See all related articles

Animal cells regulate volume via regulatory volume decrease (RVD) involving KCl efflux. This study reveals how purinergic and Ca2+-sensing receptors modulate RVD and apoptotic volume decrease (AVD) through shared signaling pathways.

Area of Science:

  • Cell Biology
  • Physiology
  • Molecular Biology

Background:

  • Animal cells possess a fundamental ability to regulate their volume, particularly under osmotic stress.
  • Regulatory volume decrease (RVD) is a key mechanism involving KCl efflux through activated K+ and Cl- channels.
  • Apoptotic volume decrease (AVD) is linked to cell death initiation and involves normotonic cell shrinkage.

Purpose of the Study:

  • To investigate the molecular mechanisms of RVD in human epithelial cells (Intestine 407).
  • To explore the roles of purinergic ATP (P2Y2) receptors and Ca2+-sensing receptors (CaR) in RVD.
  • To elucidate the relationship between AVD, RVD, and shared signaling pathways in cell volume regulation and survival.

Main Methods:

  • Studied RVD in Intestine 407 cells under osmotic stress.

Related Experiment Videos

  • Investigated the impact of ATP release and purinergic receptor stimulation on RVD.
  • Examined the role of CaR in modulating volume-sensitive Cl- channels.
  • Analyzed AVD induction by death receptors in various cell lines (U937, HeLa, NG108-15, PC12).
  • Utilized blockers of volume-regulatory K+ and Cl- channels to assess AVD mechanisms.

    • Main Results:

    • Osmotic swelling increased cytosolic Ca2+, activating IK channels and inducing ATP release.
    • Released ATP stimulated P2Y2 receptors, enhancing RVD via Ca2+ mobilization and IK channel augmentation.
    • CaR stimulation augmented volume-sensitive outwardly rectifying Cl- channels, facilitating RVD.
    • AVD induction by death receptors was observed in multiple cell types and facilitated RVD.
    • Blockade of volume-regulatory ion channels inhibited AVD and subsequent apoptotic events.

    Conclusions:

    • RVD is facilitated by P2Y2 receptor stimulation and CaR activation, involving Ca2+ mobilization and augmentation of specific ion channels.
    • AVD shares common downstream signaling pathways with RVD, suggesting a link between volume regulation and cell death.
    • Understanding these receptor-mediated processes provides insights into cell volume control and survival mechanisms.