Related Experiment Video
Updated: Aug 15, 2026
Three-Dimensional (3D) Tumor Spheroid Invasion Assay
Published on: May 1, 2015
A novel CD44 v3 isoform is involved in head and neck squamous cell carcinoma progression
E J Franzmann1, D T Weed, F J Civantos
1Department of Otolaryngology, School of Medicine, University of Miami, FL 33101, USA. efranzman@med.miami.edu
Objectives:
CD44 comprises a family of isoforms involved in tumorigenesis. Here we investigate the role of CD44 isoforms in head and neck squamous cell carcinoma (HNSCC) progression.
Materials And Methods:
HNSCC specimens underwent reverse transcriptase-polymerase chain reaction (RT-PCR) followed by Southern blot analysis. After surface biotinylation, FaDu (hypopharyngeal HNSCC) and CD44v3-transfected COS-7 cells were CD44 antibody-precipitated and compared by Western blot analysis. FaDu cells underwent double immunofluorescence staining and growth assays.
Results:
Southern blot analysis suggested differential CD44v3 isoform expression in tumor and normal tissue. Cloning and sequencing revealed 2 novel CD44v isoforms. Western blot analysis suggested CD44v3 expression in COS-7 transfectants and FaDu. Double immunofluorescence staining revealed co-localization of CD44v3 and actin in FaDu projections. Anti-CD44v3 antibody decreased FaDu growth.
Conclusion:
HNSCC tissue and FaDu appear to express CD44v3 isoforms. These isoforms may promote tumorigenesis.
Clinical Significance:
CD44v3 isoforms may be effective tumor markers and targets for HNSCC therapy.
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