Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mosquito cells bind and replicate hepatitis C virus.

R Germi1, J M Crance, D Garin

  • 1Laboratoire de Virologie Moléculaire et Structurale EA2939, Faculté de Médecine/Pharmacie de Grenoble, France.

Journal of Medical Virology
|April 4, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inter-laboratory variability in cytomegalovirus DNA quantification: implications for standardization and clinical monitoring.

Journal of clinical microbiology·2025
Same author

Human endogenous retroviral protein triggers deficit in glutamate synapse maturation and behaviors associated with psychosis.

Science advances·2020
Same author

Gap suppression at a Lifshitz transition in a multi-condensate superconductor.

Nature materials·2019
Same author

Competition between electron pairing and phase coherence in superconducting interfaces.

Nature communications·2018
Same author

Decrease of blood anti-α1,3 Galactose Abs levels in multiple sclerosis (MS) and clinically isolated syndrome (CIS) patients.

Clinical immunology (Orlando, Fla.)·2017
Same author

New diketopiperazines as vectors for peptide protection and brain delivery: Synthesis and biological evaluation.

Journal of labelled compounds & radiopharmaceuticals·2016

Hepatitis C virus (HCV) can bind to various cell types. However, only mosquito AP61 and African green monkey Vero cells demonstrated significant HCV replication and infectious virus production in this study.

Area of Science:

  • Virology
  • Cell Biology

Background:

  • Hepatitis C virus (HCV) replication has been observed in certain lymphocyte and hepatocyte cell lines.
  • Identifying additional cell lines that support HCV binding and replication is crucial for research.

Purpose of the Study:

  • To identify and evaluate new cell lines capable of binding and replicating the hepatitis C virus.
  • To investigate the role of CD81 molecules in HCV binding to different cell types.

Main Methods:

  • Human hepatoma PLC/PRF/5, human lymphoma Namalwa, African green monkey Vero, and mosquito AP61 cells were exposed to HCV-positive plasma.
  • HCV presence was assessed using RT-PCR, and binding was confirmed by RNA detection post-inoculation and in washes.
  • Successive virus passages were performed to confirm sustained replication.

Related Experiment Videos

Main Results:

  • All tested cell lines demonstrated the ability to bind HCV.
  • HCV replication and production of infectious virus were evident only in AP61 and Vero cells over 28 days and four passages.
  • Vero cells expressed CD81 molecules, a known HCV receptor, while AP61 cells did not.

Conclusions:

  • Mosquito AP61 and African green monkey Vero cells are suitable models for studying hepatitis C virus replication.
  • The findings suggest that receptors other than CD81 may mediate HCV binding to AP61 and Vero cells.