Related Experiment Video
Updated: Oct 1, 2026
Analysis of Schwann-astrocyte Interactions Using In Vitro Assays
Published on: January 13, 2011
[The influence of interleukin-1 on nerve growth factor secretion in newborn rat astrocytes in vitro]
Objective:
To study the nerve growth factor (NGF) expression and the influence of IL-1 alpha or IL-1 beta on NGF secretion in newborn rat astrocytes.
Methods:
Astrocytes obtained from the brain cortex of newborn rats were cultured and purified, and they were divided into three groups, experimental, control and blank groups. IL-1 alpha or IL-1 beta were added into the experimental group with 25, 50 and 100 U/ml, each group was cultured for 24, 48 or 72 hours, and then the NGF contents in cultured astrocytes suspension media were measured by a two-cite enzymelinked immunoserbent assay (ELISA).
Results:
Astrocytes could secret NGF by themselves and each concentration of IL-1 alpha or IL-1 beta media at any testing time could enhance NGF secreting in newborn rat astrocytes in certain degrees. The effects of IL-1 beta were stronger than IL-1 alpha, the strongest effect in the unit time was observed in IL-1 beta with 50 U/ml for 24 hours.
Conclusion:
Astrocytes can express NGF, and IL-1 alpha or IL-1 beta can enhance the NGF expression in newborn rat astrocytes.
