Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid and sensitive biosensor for Salmonella.

S T Pathirana1, J Barbaree, B A Chin

  • 1Institute for Biological Detection Systems, Department of Anatomy, Physiology and Pharmacology, Auburn University, Alabama, 36849, USA.

Biosensors & Bioelectronics
|April 5, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevention of excessive exercise-induced adverse effects in rats with Bacillus subtilis BSB3.

Journal of applied microbiology·2019
Same author

Yeast fermentate prebiotic improves intestinal barrier integrity during heat stress by modulation of the gut microbiota in rats.

Journal of applied microbiology·2019
Same author

Thermodynamic evaluation of vesicles shed by erythrocytes at elevated temperatures.

Colloids and surfaces. B, Biointerfaces·2015
Same author

Oral administration of Bacillus subtilis strain BSB3 can prevent heat stress-related adverse effects in rats.

Journal of applied microbiology·2014
Same author

Mechanism of killing of spores of Bacillus anthracis in a high-temperature gas environment, and analysis of DNA damage generated by various decontamination treatments of spores of Bacillus anthracis, Bacillus subtilis and Bacillus thuringiensis.

Journal of applied microbiology·2013
Same author

Two simple cryostats for low temperature fluorescence and absorption spectroscopy.

Applied optics·2010

A new sensor detects Salmonella typhimurium rapidly and sensitively using acoustic wave technology. This biosensor offers a detection limit of a few hundred cells/ml and maintains sensitivity for over 30 days.

Area of Science:

  • Biosensor technology
  • Acoustic wave devices
  • Immunological detection methods

Background:

  • Salmonella typhimurium poses a significant public health risk.
  • Accurate and rapid detection methods are crucial for food safety and clinical diagnostics.
  • Existing detection methods can be time-consuming or lack sensitivity.

Purpose of the Study:

  • To develop a rapid and sensitive biosensor for Salmonella typhimurium detection.
  • To utilize acoustic wave device technology for bacterial quantification.
  • To immobilize polyclonal antibodies for specific bacterial capture.

Main Methods:

  • Immobilization of polyclonal antibodies onto a quartz crystal acoustic wave device surface using the Langmuir-Blodgett method.
  • Quantification of bacterial binding by measuring changes in crystal resonance parameters via output voltage.

Related Experiment Videos

  • Testing sensor performance across a range of bacterial concentrations.

    • Main Results:

    • Achieved a lower detection limit of a few hundred cells/ml for Salmonella typhimurium.
    • Demonstrated a rapid response time of less than 100 seconds.
    • Observed a linear sensor response between 10(2)-10(7) cells/ml with a sensitivity of 18 mV/decade.
    • Confirmed specific bacterial binding, requiring two binding sites per cell.
    • Maintained approximately 75% of sensor sensitivity over 32 days.

    Conclusions:

    • The developed acoustic wave biosensor provides a rapid, sensitive, and specific method for Salmonella typhimurium detection.
    • The Langmuir-Blodgett immobilization technique enhances sensor stability and performance.
    • This technology holds promise for real-time monitoring and early detection of bacterial contamination.