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Related Experiment Videos

Rapid and sensitive biosensor for Salmonella.

S T Pathirana1, J Barbaree, B A Chin

  • 1Institute for Biological Detection Systems, Department of Anatomy, Physiology and Pharmacology, Auburn University, Alabama, 36849, USA.

Biosensors & Bioelectronics
|April 5, 2001
PubMed
Summary
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A new sensor detects Salmonella typhimurium rapidly and sensitively using acoustic wave technology. This biosensor offers a detection limit of a few hundred cells/ml and maintains sensitivity for over 30 days.

Area of Science:

  • Biosensor technology
  • Acoustic wave devices
  • Immunological detection methods

Background:

  • Salmonella typhimurium poses a significant public health risk.
  • Accurate and rapid detection methods are crucial for food safety and clinical diagnostics.
  • Existing detection methods can be time-consuming or lack sensitivity.

Purpose of the Study:

  • To develop a rapid and sensitive biosensor for Salmonella typhimurium detection.
  • To utilize acoustic wave device technology for bacterial quantification.
  • To immobilize polyclonal antibodies for specific bacterial capture.

Main Methods:

  • Immobilization of polyclonal antibodies onto a quartz crystal acoustic wave device surface using the Langmuir-Blodgett method.
  • Quantification of bacterial binding by measuring changes in crystal resonance parameters via output voltage.

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  • Testing sensor performance across a range of bacterial concentrations.

    • Main Results:

    • Achieved a lower detection limit of a few hundred cells/ml for Salmonella typhimurium.
    • Demonstrated a rapid response time of less than 100 seconds.
    • Observed a linear sensor response between 10(2)-10(7) cells/ml with a sensitivity of 18 mV/decade.
    • Confirmed specific bacterial binding, requiring two binding sites per cell.
    • Maintained approximately 75% of sensor sensitivity over 32 days.

    Conclusions:

    • The developed acoustic wave biosensor provides a rapid, sensitive, and specific method for Salmonella typhimurium detection.
    • The Langmuir-Blodgett immobilization technique enhances sensor stability and performance.
    • This technology holds promise for real-time monitoring and early detection of bacterial contamination.