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Updated: Oct 8, 2026

Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology
Published on: May 31, 2022
Heme oxygenase-1 induction by nitric oxide in RAW 264.7 macrophages is upregulated by a cyclo-oxygenase-2 inhibitor
M J Alcaraz1, A Habib, C Créminon
1Department of Pharmacology, University of Valencia, Spain. maria.j.alcarez@uv.es
Abstract:
Unstimulated RAW 264.7 macrophages express negligible heme oxygenase-1 (HO-1) protein but incubation with the nitric oxide (NO) donor spermine nonoate (SPNO) induced HO-1 and weakly cyclo-oxygenase-2 (COX-2) protein. This effect was potentiated by coincubation with the COX-2 selective inhibitor, SC58125. Cells incubated with SPNO showed a strong increase in HO-1 mRNA levels after 4 h with a significant potentiation in the presence of SC58125, which did not modify HO-1 mRNA stability. The induction of HO-1 by NO and its potentiation by anti-inflammatory agents may play a role in inflammatory and immune responses.
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