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Updated: Oct 8, 2026

Gene Trapping Using Gal4 in Zebrafish
Published on: September 29, 2013
tRNA genes were found in Piscirickettsia salmonis 16S-23S rDNA spacer region (ITS)
A Casanova1, J Obreque, A M Sandino
1Laboratorio de Virología, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Alameda 3363, Santiago-Chile, Casilla 40 Correo 33, Santiago-Chile, Chile.
Abstract:
Piscirickettsia salmonis is the etiological agent of Salmonid Rickettsial Septicemia, a disease affecting salmon aquaculture industry. We analyzed the 16S-23S rDNA spacer region (internal transcribed spacer, ITS) of Chilean P. salmonis isolates LF-89 and EM-90. Two main ITS amplification products were obtained by PCR using L1 and G1 primers, differing from that described where only one ITS region was found. By Southern blot, it was established that these two amplification products contained sequences related to P. salmonis ITS. Sequence analysis confirmed that P. salmonis had two ITS regions: ITS A and ITS B. In both isolates, the smaller (ITS B) corresponded to ITS sequences previously described for each one, and the larger (ITS A) were almost the same as their respective ITS B sequences interrupted by an insert which contained two tRNAs genes: tRNA-Ile and tRNA-Ala.
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