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FlhD/FlhC-regulated promoters analyzed by gene array and lacZ gene fusions
B M Prüss1, X Liu, W Hendrickson
1Department of Microbiology and Immunology (M/C 790), College of Medicine, University of Illinois at Chicago, E-603 Medical Sciences Building, 835 S. Wolcott, Chicago, IL 60612-7344, USA. pruess@uic.edu
Abstract:
The Escherichia coli transcriptional regulatory complex FlhD/FlhC, initially identified as a flagella-specific activator, is a global regulator involved in many cellular processes. Using gene arrays, lacZ gene fusions and enzyme assays, eight new targets of FlhD/FlhC were recognized. These are the transporter for galactose (MglBAC), the rod-shape determination proteins (MreBCD), malate dehydrogenase, and several enzymes involved in anaerobic respiration (glycerol 3-phosphate dehydrogenase, GlpABC; periplasmic nitrate reductase, NapFAGHBC; nitrite reductase, NrfABCDEFG; dimethyl sulfoxide reductase, DmsABC; and the modulator for hydrogenases, HydNHypF).