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Fibrin induces IL-8 expression from human oral squamous cell carcinoma cells
R V Lalla1, S J Goralnick, M L Tanzer
1Department of Oral Diagnosis, Division of Oral Medicine, University of Connecticut School of Dental Medicine, Farmington, CT 06030, USA.
Oral Oncology
|April 5, 2001
Summary
Fibrin directly stimulates Interleukin-8 (IL-8) expression in oral squamous cell carcinoma (OSCC) cells, promoting tumor angiogenesis. This finding suggests fibrin plays a key role in oral cancer progression by upregulating IL-8.
Area of Science:
- Oncology
- Biochemistry
- Cell Biology
Background:
- Fibrin has been observed in association with oral squamous cell carcinoma (OSCC) cells in vivo.
- Tumor-associated fibrin may influence the expression of angiogenic factors by oral tumor cells.
Purpose of the Study:
- To investigate whether fibrin can directly induce the expression of Interleukin-8 (IL-8), a major angiogenic factor, from OSCC cells in vitro.
- To establish the specificity of fibrin's effect on IL-8 expression compared to other matrix proteins.
Main Methods:
- Oral and pharyngeal tumor cell lines and a non-tumorigenic oral cell line were cultured in vitro.
- Cells were treated with varying concentrations and durations of fibrin, fibrinogen, thrombin, and collagen.
- Interleukin-8 (IL-8) expression was quantified using ELISA, with molecular identity confirmed by gel filtration chromatography.
Main Results:
- A physiologically relevant concentration of fibrin dose- and time-dependently stimulated IL-8 expression in OSCC cells.
- Fibrin did not induce significant IL-8 expression in a non-tumorigenic oral cell line.
- Fibrinogen, thrombin, and collagen did not induce significant IL-8 expression, indicating fibrin specificity.
Conclusions:
- Fibrin directly upregulates IL-8 expression in oral tumor cells.
- This fibrin-induced IL-8 production may promote angiogenesis in oral squamous cell carcinoma.
- Fibrin represents a potential therapeutic target for modulating angiogenesis in oral cancers.