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Related Experiment Videos

Gadd45gamma is dispensable for normal mouse development and T-cell proliferation.

A Hoffmeyer1, R Piekorz, R Moriggl

  • 1Howard Hughes Medical Institute, Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.

Molecular and Cellular Biology
|April 5, 2001
PubMed
Summary

Growth arrest and DNA damage-inducible gene family 45 gamma (Gadd45gamma) is induced by interleukin-2 (IL-2) but is not essential for mouse development or T-cell responses, suggesting functional redundancy.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Gadd45gamma is induced by IL-2 in T cells, unlike other Gadd45 family members.
  • Previous studies suggested Gadd45gamma's role in negative growth control.
  • The IL-2-induced expression relies on Jak3 and Stat5 signaling pathways.

Purpose of the Study:

  • To investigate the physiological role of Gadd45gamma in vivo.
  • To determine if Gadd45gamma is essential for normal development, hematopoiesis, and T-cell function.

Main Methods:

  • Generated Gadd45gamma-deficient mice using homologous recombination.
  • Assessed development, fertility, hematopoiesis, and T-cell responses to IL-2 in knockout mice.

Main Results:

Related Experiment Videos

  • Gadd45gamma-deficient mice exhibit normal development, fertility, and hematopoiesis.
  • Gadd45gamma-deficient T lymphocytes display normal responses to IL-2.
  • Gadd45gamma is dispensable for IL-2-induced T-cell proliferation.

Conclusions:

  • Gadd45gamma is not essential for normal mouse development and hematopoiesis.
  • Functional redundancy among Gadd45 family members may explain the lack of phenotype.
  • Gadd45gamma plays a dispensable role in IL-2-mediated T-cell proliferation.