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Gadd45gamma is dispensable for normal mouse development and T-cell proliferation
A Hoffmeyer1, R Piekorz, R Moriggl
1Howard Hughes Medical Institute, Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Molecular and Cellular Biology
|April 5, 2001
Summary
Growth arrest and DNA damage-inducible gene family 45 gamma (Gadd45gamma) is induced by interleukin-2 (IL-2) but is not essential for mouse development or T-cell responses, suggesting functional redundancy.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Gadd45gamma is induced by IL-2 in T cells, unlike other Gadd45 family members.
- Previous studies suggested Gadd45gamma's role in negative growth control.
- The IL-2-induced expression relies on Jak3 and Stat5 signaling pathways.
Purpose of the Study:
- To investigate the physiological role of Gadd45gamma in vivo.
- To determine if Gadd45gamma is essential for normal development, hematopoiesis, and T-cell function.
Main Methods:
- Generated Gadd45gamma-deficient mice using homologous recombination.
- Assessed development, fertility, hematopoiesis, and T-cell responses to IL-2 in knockout mice.
Main Results:
- Gadd45gamma-deficient mice exhibit normal development, fertility, and hematopoiesis.
- Gadd45gamma-deficient T lymphocytes display normal responses to IL-2.
- Gadd45gamma is dispensable for IL-2-induced T-cell proliferation.
Conclusions:
- Gadd45gamma is not essential for normal mouse development and hematopoiesis.
- Functional redundancy among Gadd45 family members may explain the lack of phenotype.
- Gadd45gamma plays a dispensable role in IL-2-mediated T-cell proliferation.
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