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Related Experiment Videos

Minimizing analyte electrolysis in an electrospray emitter.

V Kertesz1, G J Van Berkel

  • 1Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37381-6365, USA.

Journal of Mass Spectrometry : JMS
|April 5, 2001
PubMed
Summary

Using fused silica capillaries in electrospray (ES) ion sources minimizes analyte electrolysis. This modification maintains ion signal for non-electroactive analytes and protects electroactive analytes during mass spectrometry.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Electrochemistry

Background:

  • Electrospray (ES) ion sources function as controlled-current electrolytic flow cells.
  • Electrolytic reactions within the ES emitter capillary are crucial for generating charged droplets and gas-phase ions.
  • Analyte participation in these electrolytic processes can alter experimental outcomes.

Purpose of the Study:

  • To investigate a method for minimizing analyte electrolysis in electrospray ionization.
  • To assess the impact of emitter material on analyte stability during ES-MS.
  • To maintain analytical performance while enhancing analyte protection.

Main Methods:

  • Comparison of metal emitter capillaries versus fused silica capillaries in commercial ES sources.

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  • Evaluation of gas-phase ion signal generation for non-electroactive analytes.
  • Assessment of electroactive analyte oxidation under varying flow rates and electrolyte concentrations.

    • Main Results:

    • Replacing metal capillaries with fused silica effectively minimizes analyte electrolysis.
    • The modified ES system maintains comparable gas-phase ion signal for non-electroactive analytes.
    • Minimal oxidation of electroactive analytes was observed even at low flow rates and high electrolyte concentrations.

    Conclusions:

    • Fused silica capillaries offer a simple and effective solution to mitigate analyte electrolysis in ES ion sources.
    • This modification preserves the analytical utility of ES-MS for a wide range of analytes.
    • The use of fused silica capillaries enhances the reliability of ES-MS for studying electroactive species.