Resorption and calcification of chemically modified collagen/hyaluronan hybrid membranes

The objectives of this study were to characterize collagen/hyaluronan hybrid membrane before and after different chemical crosslinking, as well as to investigate the impact of the treatment method on resorption and calcification processes by in vivo tests. The kinetics study of resorption of collagen/hyaluronan membranes showed that after 28 days the membranes were completely resorbed. The modification of the membranes with glutaraldehyde comparing to the similar modification of pericardial tissue conducts higher calcification. Treatment with hexamethylene diisocyanate together with poly(oxyethylene) improved mechanical properties but the resorption process was similar. Additional treatments with diphenylphosphorylazide and 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide (EDAC) resulted in prolongation of the resorption time. Calcification was suppressed in case of treatment with EDAC.