Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electrophoretic injection bias in a microchip valving scheme.

J P Alarie1, S C Jacobson, J M Ramsey

  • 1Oak Ridge National Laboratory, TN 37831-6142, USA.

Electrophoresis
|April 6, 2001
PubMed
Summary

The pinched injection strategy on microfluidic chips causes bias, favoring neutral species over anionic ones during sample loading and dispensing. This leads to significant differences in injected analyte volumes, impacting electrophoretic separations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inferring distributional shifts of epidemiologically important North and Central American sandflies from Pleistocene to future scenarios.

Medical and veterinary entomology·2018
Same author

Behavioural and electrophysiological responses of Triatoma dimidiata nymphs to conspecific faecal volatiles.

Medical and veterinary entomology·2017
Same author

Impact of climate change on vector transmission of Trypanosoma cruzi (Chagas, 1909) in North America.

Medical and veterinary entomology·2017
Same author

Towards a paradigm shift in the treatment of chronic Chagas disease.

Antimicrobial agents and chemotherapy·2013
Same author

Carbon monoxide exposures in parking garages.

Bulletin of environmental contamination and toxicology·2013
Same author

Volatile compounds emitted by Triatoma dimidiata, a vector of Chagas disease: chemical analysis and behavioural evaluation.

Medical and veterinary entomology·2012

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • Electrophoretic separation techniques are crucial for analyzing complex mixtures.
  • Microfluidic devices offer miniaturized platforms for various analytical applications.
  • Injection bias in capillary electrophoresis (CE) and microchip electrophoresis (ME) can significantly affect quantitative accuracy.

Purpose of the Study:

  • To investigate the electrophoretic injection bias associated with the pinched injection strategy on microfluidic devices.
  • To identify the specific steps within the pinched injection process that contribute to the observed bias.
  • To quantify the extent of bias for neutral versus anionic species.

Main Methods:

  • Utilized microfabricated fluidic devices (microchips) for implementing the pinched injection strategy.

Related Experiment Videos

  • Employed fluorescently labeled amino acids as model analytes to represent neutral and anionic species.
  • Analyzed sample loading and dispensing steps to determine their contribution to injection bias.

    • Main Results:

    • The pinched injection strategy exhibited a bias, preferentially injecting neutral species over anionic species.
    • Both sample loading and dispensing steps were identified as contributors to this injection bias.
    • Neutral species occupied a larger volume at the cross-intersection during loading and were injected in greater volumes during dispensing compared to anionic species.
    • Observed differences in injected volumes between neutral and anionic analytes reached up to 27%.

    Conclusions:

    • The pinched injection strategy on microfluidic chips is susceptible to injection bias, favoring neutral analytes.
    • Understanding and mitigating this bias in both loading and dispensing is critical for accurate quantitative analysis using microchip electrophoresis.
    • Further optimization of injection protocols is necessary to minimize bias and improve the reliability of microfluidic separation systems.