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Updated: Jul 16, 2026
07:59
Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
Published on: July 21, 2017
In normal men, free fatty acids reduce peripheral but not splanchnic glucose uptake
V Rigalleau1, C Binnert, K Minehira
1Institut de Physiologie, Lausanne, Switzerland. vincent.rigalleau@iphysiol.unil.ch
Diabetes
|April 6, 2001
Summary
Elevated free fatty acids (FFAs) impair peripheral glucose uptake but not splanchnic glucose uptake. FFAs increase total glucose output, independent of glucose-6-phosphatase stimulation.
Area of Science:
- Metabolic research
- Endocrinology
- Physiology
Background:
- Plasma free fatty acid (FFA) elevation is linked to impaired glucose metabolism.
- Understanding FFAs' impact on splanchnic glucose uptake and output is crucial for elucidating lipid-induced glucose intolerance.
Purpose of the Study:
- To investigate the effects of elevated FFAs on splanchnic glucose uptake, total glucose output, and glucose cycling during hyperinsulinemic-hyperglycemic conditions.
Main Methods:
- Utilized a hyperinsulinemic-hyperglycemic clamp with [2H7]glucose infusion to measure glucose turnover and cycling rates in eight healthy volunteers.
- Administered lipid or saline infusions, with a subset receiving glycerol infusion, before and after oral glucose load.
Main Results:
- Lipid infusion did not reduce first-pass splanchnic glucose uptake but decreased peripheral glucose uptake by 40% (P < 0.01).
- Total glucose output significantly increased with FFAs post-oral glucose ingestion (P < 0.01 vs. saline).
- Glucose cycling rates did not significantly increase and tended to decrease during lipid infusion.
Conclusions:
- In moderate hyperinsulinemia-hyperglycemia, FFAs reduce peripheral glucose uptake while sparing splanchnic glucose uptake.
- Elevated FFAs increase total glucose output through a mechanism not involving glucose-6-phosphatase stimulation.
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