Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single bead characterization using analytical constructs: application to quality control of libraries.

O Lorthioir1, R A Carr, M S Congreve

  • 1Glaxo SmithKline Medicines Research Centre, Stevenage, Hertfordshire, UK. o165917@gsk.com

Analytical Chemistry
|April 6, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognosis after neoadjuvant chemoradiation or chemotherapy for locally advanced gastro-oesophageal junctional adenocarcinoma.

The British journal of surgery·2021
Same author

Mitochondrial DNAs provide insight into trypanosome phylogeny and molecular evolution.

BMC evolutionary biology·2020
Same author

Pruritic eruption in a patient with end-stage renal disease.

Clinical and experimental dermatology·2020
Same author

PRAME immunohistochemistry as an adjunct in the diagnosis of paucicellular lentigo maligna in a young man.

The British journal of dermatology·2020
Same author

A common cause of hair loss?

Clinical and experimental dermatology·2020
Same author

A pruritic hyperkeratotic plaque on the foot of a 35-year-old woman.

Clinical and experimental dermatology·2020

Analytical construct technology enables sensitive single-bead analysis of combinatorial libraries. This method improves quality control by generating analytical fragments detectable by electrospray mass spectrometry (ESI-MS), even for difficult-to-see compounds.

Area of Science:

  • Chemical synthesis
  • Analytical chemistry
  • Biotechnology

Background:

  • Combinatorial libraries are crucial for drug discovery.
  • Current quality control methods for single-bead analysis can be insensitive.
  • Detecting all synthesized compounds in a library is challenging.

Purpose of the Study:

  • To apply analytical construct technology for enhanced single-bead analysis.
  • To develop a more sensitive method for quality control of combinatorial libraries.
  • To improve the detection of compounds in split-mix libraries.

Main Methods:

  • Utilizing analytical construct technology with orthogonal cleavage.
  • Generating an analytical fragment incorporating the substrate.
  • Employing isotope labeling for enhanced electrospray mass spectrometry (ESI-MS) detection.

Related Experiment Videos

  • Performing 1H NMR for conventional cleavage validation.

    • Main Results:

    • Analytical construct technology successfully enabled single-bead analysis.
    • Orthogonal cleavage produced highly ESI-MS-sensitized analytical fragments.
    • Compounds undetectable by conventional methods were visualized using this new approach.
    • 1H NMR confirmed the presence and purity of conventionally cleaved compounds.

    Conclusions:

    • Analytical construct technology offers a significant improvement for quality control in combinatorial chemistry.
    • This method enhances the sensitivity and scope of single-bead analysis.
    • The approach addresses current limitations in detecting and analyzing library compounds.