Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Anergy induction by dimeric TCR ligands.

H Appel1, N P Seth, L Gauthier

  • 1Department of Cancer Immunology & AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|April 6, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Chronic rhinosinusitis with nasal polyposis : A retrospective analysis of therapeutic approaches in 463 patients].

HNO·2024
Same author

Chemical Responses of Nicotiana tabacum (Solanaceae) Induced by Vibrational Signals of a Generalist Herbivore.

Journal of chemical ecology·2019
Same author

Performance of the ATLAS trigger system in 2015.

The European physical journal. C, Particles and fields·2017
Same author

Searches for pair production of third-generation squarks in [Formula: see text][Formula: see text] pp collisions.

The European physical journal. C, Particles and fields·2017
Same author

Reconstruction of primary vertices at the ATLAS experiment in Run 1 proton-proton collisions at the LHC.

The European physical journal. C, Particles and fields·2017
Same author

Measurement of the top quark mass using single top quark events in proton-proton collisions at [Formula: see text] TeV.

The European physical journal. C, Particles and fields·2017

Researchers created a soluble dimeric DR2-IgG fusion protein to target T cells. This approach induced T cell anergy, a state of unresponsiveness, offering potential therapeutic strategies for autoimmune diseases like multiple sclerosis.

Area of Science:

  • Immunology
  • Autoimmune Diseases
  • T cell biology

Background:

  • Self-antigen recognition by T cells is implicated in autoimmune disease pathogenesis.
  • Multiple sclerosis susceptibility is linked to HLA-DR2, which presents myelin basic protein peptides to CD4(+) T cells.

Purpose of the Study:

  • To develop targeted therapies for autoimmune diseases by inducing T cell anergy.
  • To investigate the potential of soluble dimeric MHC class II/peptide complexes in modulating T cell responses.

Main Methods:

  • Expression of a soluble dimeric DR2-IgG fusion protein complexed with a myelin basic protein (MBP) peptide.
  • Activation of MBP-specific T cells using the engineered DR2/MBP complexes.
  • Assessment of T cell responsiveness and viability following initial TCR signaling.

Related Experiment Videos

Main Results:

  • Soluble, dimeric DR2/MBP peptide complexes activated MBP-specific T cells without costimulatory or adhesion signals.
  • Initial TCR signaling induced T cell anergy, rendering them unresponsive to subsequent activation.
  • Anergic T cells, initially viable, became susceptible to late apoptosis due to insufficient cytokine production.

Conclusions:

  • Dimerization of T cell receptors (TCRs) with bivalent MHC class II/peptide complexes can induce anergy in human CD4(+) T cells.
  • This approach allows for the induction of T cell anergy with defined MHC/peptide specificity.
  • Targeted induction of T cell anergy presents a potential therapeutic strategy for autoimmune conditions.