[Phosphorus metabolism in yeasts, oxidizing hydrocarbons during active aeration of the medium]

Variations in the phosphorus and nitrogen assimilation by hydrocarbon oxidizing yeast C. guilliermondii K-5 were followed upon an active aeration of the medium Changes in the phosphate forms of the acid-soluble and acid-insoluble fractions were found. By the end of the lag-phase the content of all phosphate forms, except stable polyphosphates, increased. At the onset of the active budding the content of labile phosphates, belonging to acid-soluble phosphates, increased. As the culture developed, the content of these forms decreased and increased slightly in the growth phase with a negative acceleration. Acid-insoluble polyphosphates were accumulated in the culture as long as it grew. In the growth phase with a negative acceleration these polyphosphates were hydrolyzed. This was responsible for an increment of all phosphates in the acid-soluble fraction. The same pattern occurred in the dying phase of the culture.