Rapid detection of the 22q11.2 deletion with quantitative real-time PCR

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Serum and Plasma Copy Number Detection Using Real-time PCR
09:21

Serum and Plasma Copy Number Detection Using Real-time PCR

Published on: December 15, 2017

H Kariyazono1, T Ohno, K Ihara

  • 1Department of Pediatrics, Graduate School of Medical Sciences, Fukuoka, Japan. hide-k@mailserver.med.kyushu-u.ac.jp

Molecular and Cellular Probes
|April 9, 2001
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...