Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

VEGFR3 gene structure, regulatory region, and sequence polymorphisms.

K Iljin1, M J Karkkainen, E C Lawrence

  • 1Molecular/Cancer Biology Laboratory, Haartman Institute, University of Helsinki, 00014 Helsinki, Finland.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|April 9, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Counteracting age-related VEGF signaling insufficiency promotes healthy aging and extends life span.

Science (New York, N.Y.)·2021
Same author

Layered material platform for surface plasmon resonance biosensing.

Scientific reports·2020
Same author

Ischemia-Reperfusion Injury Enhances Lymphatic Endothelial VEGFR3 and Rejection in Cardiac Allografts.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

Donor Heart Treatment With COMP-Ang1 Limits Ischemia-Reperfusion Injury and Rejection of Cardiac Allografts.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

Lack of cardiac and high-fat diet induced metabolic phenotypes in two independent strains of Vegf-b knockout mice.

Scientific reports·2014
Same author

Identification of 33 candidate oncogenes by screening for base-specific mutations.

British journal of cancer·2014

Vascular endothelial growth factor receptor 3 (VEGFR-3) gene regulation was studied to understand its role in lymphatic development. The promoter drives endothelial cell expression, offering insights into lymphatic disorders.

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Vascular endothelial growth factor receptor 3 (VEGFR-3) is crucial for cardiovascular development and lymphatic endothelial cell function in adults.
  • Mutations in VEGFR3 are linked to hereditary lymphedema, highlighting its importance in lymphatic system disorders.

Purpose of the Study:

  • To investigate the regulatory elements controlling the lineage-restricted expression of the VEGFR3 gene.
  • To characterize the VEGFR3 gene structure and its 5' flanking regulatory region.

Main Methods:

  • Gene sequencing and characterization of the human VEGFR3 gene, including its 31 exons and alternative splicing.
  • Analysis of the proximal promoter region for sequence homology with the mouse Vegfr3 promoter.
  • In vitro transfection experiments using cultured cells to assess promoter activity.

Related Experiment Videos

  • In vivo studies using transgenic mouse embryos to evaluate promoter-driven reporter gene expression.

    • Main Results:

    • The human VEGFR3 gene comprises 31 exons with alternative splicing at exons 30a and 30b.
    • The proximal VEGFR3 promoter is TATA-less and shares sequence similarities with the mouse promoter.
    • Transfection experiments demonstrated that the Vegfr3 promoter directs endothelial cell-specific transcription.
    • In vivo studies showed weak lymphatic endothelial expression of a reporter gene driven by a 1.6 kb Vegfr3 promoter fragment in transgenic mice.

    Conclusions:

    • The proximal promoter of the VEGFR3 gene contains elements responsible for endothelial cell-specific expression.
    • Further enhancer elements likely exist elsewhere to fully regulate VEGFR3 expression.
    • The characterized VEGFR3 gene and its regulatory regions are valuable tools for studying the lymphatic system and associated diseases.