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Calprotectin expression by gingival epithelial cells.

K F Ross1, M C Herzberg

  • 1Department of Preventive Science, School of Dentistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Infection and Immunity
|April 9, 2001
PubMed
Summary
This summary is machine-generated.

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Calprotectin is constitutively expressed in gingival keratinocytes, increasing with senescence. Proinflammatory agents like LPS and IL-1 beta did not induce calprotectin expression in immortalized cells.

Area of Science:

  • Oral immunology
  • Epithelial cell biology
  • Inflammation research

Background:

  • Calprotectin, an antimicrobial protein complex of MRP8 and MRP14, is present in immune cells and oral epithelial cells.
  • Upregulation of calprotectin is observed during oral mucosal inflammation.
  • The role of epithelial cells in calprotectin expression during inflammation requires further investigation.

Purpose of the Study:

  • To investigate whether human gingival keratinocytes upregulate calprotectin expression in response to pro-inflammatory agents.
  • To determine if calprotectin expression is constitutive or inducible in oral epithelial cells.

Main Methods:

  • Primary and immortalized human gingival keratinocytes were cultured.
  • Quantitative expression of MRP8 and MRP14 mRNA was analyzed using RNase protection assays.

Related Experiment Videos

  • Calprotectin complex levels were measured by enzyme-linked immunosorbent assay.
  • Cells were treated with lipopolysaccharide (LPS), interleukin-1 beta (IL-1 beta), and phorbol myristate acetate (PMA).

    • Main Results:

    • Calprotectin mRNA and protein expression were found to be constitutive in primary gingival keratinocytes.
    • Expression of calprotectin subunits (MRP8 and MRP14) tended to increase as primary cells approached senescence.
    • Immortalized keratinocytes showed increased MRP8 and MRP14 mRNA expression upon PMA stimulation but not in response to LPS or IL-1 beta.
    • LPS, IL-1 beta, and PMA all upregulated interleukin-8 (IL-8) expression.

    Conclusions:

    • Calprotectin is constitutively expressed in cultured gingival keratinocytes.
    • Calprotectin expression in immortalized keratinocytes appears independent of exogenous pro-inflammatory agents LPS and IL-1 beta.
    • These findings suggest that while calprotectin is present in epithelial cells, its induction by common inflammatory mediators like LPS and IL-1 beta may be limited in this cell type.