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Updated: Jul 19, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
High-performance liquid chromatographic analysis for the determination of buspirone analogue--mesmar in rat plasma
K Kobylińska1, I Cendrowska, M Kobylińska
1Pharmacology Department, Pharmaceutical Research Institute, 8 Rydygiera Street, 01-793 Warsaw, Poland.
Abstract:
A simple and selective analytical method for the determination of buspirone analogue--mesmar in rat plasma is described. The procedure involves liquid-liquid extraction followed by reversed-phase high-performance chromatographic analysis with UV detection at 245 nm. No endogenous compounds were found to interfere. The absolute extraction recovery from plasma samples was 76%. The linearity was assessed in the range 0.12-2.44 nmol/ml. Stability of mesmar during processing (autosampler) and in plasma was checked. This method proved suitable for pharmacokinetic studies following single oral dose in rats.
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