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Related Experiment Videos

Heparanase expression in clinical digestive malignancies.

H Inoue1, K Mimori, T Utsunomiya

  • 1Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu 874-0838, Japan.

Oncology Reports
|April 11, 2001
PubMed
Summary

Heparanase mRNA expression varies across gastrointestinal cancers, with higher levels found in gastric cancer. This study found no significant impact on prognosis, suggesting further research is needed.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Gastroenterology

Background:

  • Heparanase, a mammalian enzyme, is implicated in tumor progression.
  • Its expression in human cancers requires further investigation.
  • Understanding heparanase expression is crucial for cancer research.

Purpose of the Study:

  • To determine heparanase mRNA expression in esophageal, gastric, and colorectal cancers.
  • To compare heparanase expression levels across these cancer types.
  • To assess the correlation between heparanase expression and clinicopathological factors.

Main Methods:

  • Analysis of heparanase mRNA expression in 30 esophageal cancer cell lines.
  • Reverse transcriptase-polymerase chain reaction (RT-PCR) on 144 clinical cancer samples (esophageal, gastric, colorectal) and paired normal tissues.

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  • Calculation of tumor-to-normal (T/N) heparanase mRNA expression ratios.

    • Main Results:

    • All 30 esophageal cancer cell lines exhibited heparanase mRNA expression.
    • Gastric cancer cases showed a significantly higher frequency of high heparanase mRNA expression compared to esophageal and colorectal cancers (p < 0.05).
    • No significant differences in clinicopathological factors or prognosis were observed between high and low heparanase expression groups within each cancer type.

    Conclusions:

    • Heparanase mRNA expression levels differ among esophageal, gastric, and colorectal cancers.
    • The clinical and pathological impact of heparanase appears limited compared to other proteinases.
    • Further studies are recommended to fully elucidate the role of heparanase in gastrointestinal malignancies.