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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

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Transformation-associated changes in gene expression of alternative splicing regulatory factors in mouse fibroblast

T Maeda1, S Furukawa

  • 1Department of Radiology and Radiation Oncology, Osaka University Graduate School of Dentistry, Osaka, Japan. tmaeda@radiol.dent.osaka-u.ac.jp

Oncology Reports
|April 11, 2001
PubMed

Insights

Altered expression of splicing regulatory factors, such as U2AF65 and SC35, is common in cellular transformation. This suggests a widespread role for splicing factor dysregulation in cancer development.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Alternative splicing is crucial in tumorigenesis, but its regulatory mechanisms are not fully understood.
  • Previous studies indicated altered splicing factor expression during chemical transformation.
  • The commonality of this phenomenon across different transformation modes remained unclear.

Purpose of the Study:

  • To investigate the expression of five key splicing regulatory factors in mouse fibroblast cell lines undergoing different transformation processes.
  • To determine if altered splicing factor expression is a common event in cellular transformation.

Main Methods:

  • Utilized clonally-derived mouse fibroblast cell lines: SV40 large T transfectants (SV-T2, NIH3T3/13C7) and c-erbB2 transfectants (A4).
  • Quantified the expression levels of heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and A2, alternative splicing factor/splicing factor 2 (ASF/SF2), spliceosome component of 35-kDa protein (SC35), and 65-kDa subunit of U2 snRNP auxiliary factor (U2AF65).

Main Results:

  • U2AF65 expression increased 2.1- and 2.7-fold in NIH3T3/13C7 and A4 cells, respectively, compared to parental cells.
  • SC35 expression showed a significant increase (1.8- to 2.3-fold) across all tested transformed cell lines.
  • hnRNP A1, hnRNP A2, and ASF/SF2 expression patterns were not detailed in the abstract.

Conclusions:

  • Altered expression of specific splicing regulatory factors, including U2AF65 and SC35, is a common occurrence during various modes of cellular transformation.
  • These findings suggest a potentially general role for dysregulated splicing factor expression in the development of cancer.

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