[Application of chromosome painting technique to analysis of structural aberration of human chromosomes]
1Department of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang 150086 P. R. China. yanghj@ems.hrbmu.edu.cn
Objective:
This study was aimed at using chromosome painting technique to detect translocation, especially microtranslocations, on chromosomes in comparison with G-banding analysis.
Methods:
Chromosome painting technique was applied to analysis of metaphase chromosomes of patients for detecting translocations with biotin-labeled chromosomes X, Y, 14q, 10 specific probes.
Results:
Fluorescence in situ hybridization FISH signals were shown clearly in slides even in specimen stored at room temperature for 10 years and at -80 centigrade degree. Translocations were located precisely.
Conclusion:
Microtranslocations, which are hard to analyze by G-banding, can be detected exactly using chromosome painting technique with G-band karyotype on metaphase chromosome.
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