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Typing of Salmonella enterica subsp. enterica serovar Mbandaka isolates
1Department of Microbiology, National Veterinary Research Institute, Partyzantów 57, 24-100 Pulawy, Poland. ahpsz@piwet.pulawy.pl
Abstract:
Recently, Salmonella enterica subsp. enterica serovar Mbandaka (S. Mbandaka) has gained some importance in the epidemiology of salmonellosis in Poland. Since biotyping, resistance typing, and plasmid profiling were insufficient for strain differentiation, genome macrorestriction by means of pulse-field gel electrophoresis (PFGE) was applied and proved to be the method of choice in S. Mbandaka epidemiological studies. XbaI and BcuI macrorestriction produced 15 and 14 pulse-field profiles (PFP), respectively, but in the case of each enzyme one profile was prevalent. When macrorestriction profiles were combined, a total 24 patterns were found. Based on the similarity of the profiles, four clonal lineages were identified. One clonal lineage contained the majority of poultry, feed and human isolates. Poultry was concluded to be an important source of S. Mbandaka for humans in Poland. Complementary use of various typing techniques improved efficacy of epidemiological studies giving possibility to subdivide S. Mbandaka into 35 types and the index of discrimination reached 0.947.
Insights
Salmonella Mbandaka (S. Mbandaka) is a growing concern in Poland. Pulse-field gel electrophoresis (PFGE) effectively distinguished strains, revealing poultry as a significant source of human infections.
Area of Science:
- Microbiology
- Epidemiology
- Genomics
Background:
- Salmonella enterica subsp. enterica serovar Mbandaka (S. Mbandaka) has emerged as a significant pathogen in Poland.
- Traditional typing methods like biotyping and plasmid profiling were inadequate for differentiating S. Mbandaka strains.
Purpose of the Study:
- To evaluate the effectiveness of genome macrorestriction using pulse-field gel electrophoresis (PFGE) for epidemiological studies of S. Mbandaka.
- To identify the sources and transmission routes of S. Mbandaka in Poland.
Main Methods:
- Genome macrorestriction using XbaI and BcuI enzymes followed by PFGE.
- Analysis of pulse-field profiles (PFPs) and combined patterns.
- Identification of clonal lineages and strain typing.
Main Results:
- PFGE with XbaI and BcuI generated 15 and 14 PFPs, respectively, with one prevalent profile for each enzyme.
- Combining profiles yielded 24 distinct patterns, leading to the identification of four clonal lineages.
- One major clonal lineage encompassed most poultry, feed, and human isolates, indicating a link between these sources.
Conclusions:
- PFGE is the method of choice for S. Mbandaka epidemiological studies, offering superior discriminatory power.
- Poultry serves as a crucial reservoir and source of S. Mbandaka infections in humans in Poland.
- Integrated typing strategies enhanced S. Mbandaka subtyping, achieving a high index of discrimination (0.947).