Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

6.3K
Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
6.3K
Pyruvate Oxidation01:15

Pyruvate Oxidation

169.2K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
169.2K
Oxidation Numbers03:14

Oxidation Numbers

42.9K
In redox reactions, the transfer of electrons occurs between reacting species. Electron transfer is described by a hypothetical number called the oxidation number (or oxidation state). It represents the effective charge of an atom or element, which is assigned using a set of rules.
42.9K
Oxidation-Reduction Reactions03:11

Oxidation-Reduction Reactions

75.8K
Oxidation–Reduction Reactions
75.8K
Stages of Infection01:26

Stages of Infection

65.4K
Stages of infection describe what happens to a susceptible host once a pathogen invades the human body. The stages of infection are incubation, prodromal, illness, stage of decline, and convalescence. The incubation stage is the period from exposure to a pathogen until symptoms start. The infected person is unaware of impending illness as the pathogens grow and multiply within the body. The duration may vary depending on the type of infection. The incubation period of measles averages ten to...
65.4K
Stages of Sleep01:22

Stages of Sleep

1.5K
Sleep progresses through distinct stages, each characterized by specific brain wave patterns and physiological responses ranging from wakefulness to stages of non-rapid eye movement, known as non-REM, to rapid eye movement, referred to as REM. Understanding these stages helps in recognizing how sleep supports various bodily and cognitive functions.
Before sleep begins, in wakefulness, the brain exhibits primarily beta waves, which are high in frequency and low in amplitude, indicating alertness...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outcomes and prognostic indicators in daratumumab-refractory multiple myeloma: a multicenter real-world study of elotuzumab, pomalidomide, and dexamethasone in 247 patients.

ESMO open·2025
Same author

Reversibility of alexithymia with effective treatment of moderate-to-severe psoriasis: longitudinal data from EPIDEPSO.

The British journal of dermatology·2018
Same author

Prevalence of alexithymia in patients with psoriasis and its association with disease burden: a multicentre observational study.

The British journal of dermatology·2016
Same author

ISOLATION OF A MONODISPERSE PROTEIN FRACTION FROM COTTONSEEDS.

Biochemical and biophysical research communications·2015
Same author

Engineering the internal cavity of neuroglobin demonstrates the role of the haem-sliding mechanism.

Acta crystallographica. Section D, Biological crystallography·2014
Same author

Efficacy and tolerability of bendamustine, bortezomib and dexamethasone in patients with relapsed-refractory multiple myeloma: a phase II study.

Blood cancer journal·2013

Related Experiment Video

Updated: Feb 10, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.8K

Nitric oxide moves myoglobin centre stage.

M Brunori

    Trends in Biochemical Sciences
    |April 11, 2001
    PubMed
    Summary
    This summary is machine-generated.

    Myoglobin (Mb) acts as a nitric oxide (NO) scavenger in muscles, not just an oxygen carrier. This study provides the first experimental evidence for Mb's novel NO scavenging function in the heart.

    More Related Videos

    Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
    08:25

    Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

    Published on: December 25, 2016

    23.1K
    Analytical Techniques for Assaying Nitric Oxide Bioactivity
    11:28

    Analytical Techniques for Assaying Nitric Oxide Bioactivity

    Published on: June 18, 2012

    18.5K

    Related Experiment Videos

    Last Updated: Feb 10, 2026

    Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
    08:23

    Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

    Published on: February 16, 2022

    4.8K
    Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
    08:25

    Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

    Published on: December 25, 2016

    23.1K
    Analytical Techniques for Assaying Nitric Oxide Bioactivity
    11:28

    Analytical Techniques for Assaying Nitric Oxide Bioactivity

    Published on: June 18, 2012

    18.5K

    Area of Science:

    • Biochemistry
    • Physiology
    • Molecular Biology

    Background:

    • Myoglobin (Mb) is traditionally known as an oxygen-binding protein in muscle tissues.
    • Nitric oxide (NO) is a signaling molecule with diverse physiological roles, including the regulation of cardiac function.
    • NO can inhibit key metabolic enzymes like cytochrome-c oxidase, impacting cellular respiration.

    Discussion:

    • This research presents the first experimental validation of myoglobin's role as a nitric oxide scavenger.
    • The study utilized perfused hearts from wild-type and myoglobin-knockout mice to demonstrate this function.
    • The findings suggest a new biochemical pathway involving myoglobin in nitric oxide homeostasis within cardiac and skeletal muscles.

    Key Insights:

    • Myoglobin actively scavenges nitric oxide in muscle tissue.
    • This NO scavenging by myoglobin has implications for understanding cardiac function and cellular respiration.
    • Oxymyoglobin exhibits high reactivity towards nitric oxide, suggesting a specific molecular mechanism.

    Outlook:

    • Further investigation into the physiological consequences of myoglobin's NO scavenging activity.
    • Exploring the therapeutic potential of modulating myoglobin-nitric oxide interactions.
    • Elucidating the precise molecular mechanisms governing the interaction between oxymyoglobin and nitric oxide.