Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transmembrane signaling in bacterial chemoreceptors.

J J Falke1, G L Hazelbauer

  • 1Department of Chemistry and Biochemistry, University of Colorado, 80309-0215, Boulder, CO, USA. falke@colorado.edu

Trends in Biochemical Sciences
|April 11, 2001
PubMed
Summary

Bacterial chemoreceptors use a piston-like helix movement to transmit signals across the cell membrane, enabling chemotaxis. This mechanism involves ligand binding and modulates histidine kinase activity for cellular response.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence that tricyclic small molecules may possess toll-like receptor and myeloid differentiation protein 2 activity.

Neuroscience·2010
Same author

Ion Channels within Ion Transport Proteins: Evidence in the Band 3 System.

Biophysical journal·2009
Same author

Evidence that both ligand binding and covalent adaptation drive a two-state equilibrium in the aspartate receptor signaling complex.

The Journal of general physiology·2001
Same author

Location of the receptor-interaction site on CheB, the methylesterase response regulator of bacterial chemotaxis.

The Journal of biological chemistry·2001
Same author

Signalling substitutions in the periplasmic domain of chemoreceptor Trg induce or reduce helical sliding in the transmembrane domain.

Molecular microbiology·2001
Same author

C2 domains from different Ca2+ signaling pathways display functional and mechanistic diversity.

Biochemistry·2001

Area of Science:

  • Microbiology
  • Biochemistry
  • Molecular Biology

Background:

  • Bacterial chemoreceptors are crucial for chemotaxis, sensing environmental chemicals.
  • They regulate noncovalently associated histidine kinases via transmembrane signaling.
  • Ligand binding initiates a signal cascade from the external domain to the internal kinase.

Purpose of the Study:

  • To investigate the mechanism of transmembrane signaling in bacterial chemoreceptors.
  • To elucidate the conformational changes involved in signal transduction.
  • To understand how chemical signals are converted into cellular responses.

Main Methods:

  • Utilizing novel strategies to probe receptor conformational changes.
  • Analyzing signaling helix movement across the receptor length (>350 Å).

Related Experiment Videos

  • Examining evidence from periplasmic and transmembrane domains.

    • Main Results:

    • Identified a subtle conformational signal within a signaling helix.
    • Demonstrated a piston-type sliding motion of the signaling helix.
    • Localized this motion to the periplasmic and transmembrane domains.

    Conclusions:

    • Bacterial chemoreceptor signaling involves a conserved piston-like helix movement.
    • This conformational change is key to transmitting signals across the membrane.
    • The mechanism explains how chemoreceptors modulate downstream kinase activity.