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Cell attachment modulation by radiation from a pulsed light diode (lambda = 820 nm) and various chemicals
T I Karu1, L V Pyatibrat, G S Kalendo
1Institute of Laser and Informatic Technologies, Russian Academy of Sciences, 142190 Troitsk, Moscow Region, Russian Federation. karu@isan.troisk.ru
Lasers in Surgery and Medicine
|April 11, 2001
Summary
Pulsed near-infrared (IR) light increases cell adhesion to glass matrices. This effect is mediated by free radical and redox processes, suggesting a role in wound repair signaling.
Area of Science:
- Cell biology
- Biophysics
- Biochemistry
Background:
- Cell-matrix interactions are crucial for wound repair.
- Understanding light's effect on cellular adhesion is key to its therapeutic applications.
Purpose of the Study:
- To investigate the mechanisms of pulsed near-infrared (IR) diode light on cellular adhesion in vitro.
- To evaluate the adhesion of HeLa cells to a glass matrix after irradiation and chemical treatments.
Main Methods:
- Irradiation of HeLa cell suspension with a pulsed near-infrared (IR) diode (820 nm).
- Treatment with various chemicals including antioxidants, respiratory chain inhibitors, and oxidative agents.
- Assessment of cell-glass adhesion using the adhesion assay technique.
Main Results:
- Pulsed IR irradiation dose-dependently increased cell-glass adhesion.
- Antioxidants and dinitrophenol (DNP) abolished the light-induced adhesion increase.
- Respiratory chain inhibitors modified the effect, while oxidative agents enhanced adhesion.
Conclusions:
- Pulsed IR radiation modulates cell-matrix attachment.
- Free radical and redox processes are involved in light-induced cell-matrix interactions.
- The observed effects suggest a transient oxidative stress in the cytoplasm.
