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Wall deactivation with fluorosurfactants for capillary electrophoretic analysis of biomolecules.

A Emmer¹, J Roeraade

¹Department of Chemistry, The Royal Institute of Technology, Stockholm, Sweden. aae@analyt.kth.se

Electrophoresis

|April 12, 2001

Summary

Fluorosurfactants improve protein and peptide separations in capillary electrophoresis by modifying capillary wall charge. This reduces protein adsorption, enhancing separation performance and enabling tailored buffer systems for specific analytical challenges.

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Area of Science:

Analytical Chemistry
Separation Science

Background:

Capillary electrophoresis (CE) is a powerful separation technique.
Protein and peptide analysis in CE can be hindered by analyte adsorption to the capillary wall.
Surface modification of capillary inner walls is crucial for improving separation efficiency.

Purpose of the Study:

To investigate the use of fluorosurfactants as buffer additives in CE for protein and peptide separation.
To explore how fluorosurfactants modify capillary surface charge and reduce analyte adsorption.
To demonstrate enhanced separation performance through optimized buffer systems.

Main Methods:

Utilizing fluorosurfactants as buffer additives in capillary electrophoresis.
Adjusting running buffer pH to control electrostatic interactions between analytes and capillary wall.
Employing mixtures of fluorosurfactants to fine-tune buffer properties.

Main Results:

Fluorosurfactants form bilayers on the capillary inner wall, enabling adjustable and reversible surface charge modification.
By matching the charge of proteins/peptides to the capillary wall, electrostatic repulsion is achieved, significantly reducing wall adsorption.
Noticeable increases in separation performance were observed, with further improvements possible using mixed fluorosurfactant systems.

Conclusions:

Fluorosurfactants are effective buffer additives for deactivating capillary walls in CE separations of proteins and peptides.
This approach enhances separation efficiency by minimizing analyte adsorption.
The buffer system can be tailored using fluorosurfactants for specific separation challenges, offering versatile applications.