Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High throughput screening: a rapid way to recombinant allergens.

R Crameri1

  • 1Swiss Institute of Allergy and Asthma Research (SIAF), Davos, Switzerland.

Allergy
|April 12, 2001
PubMed
Summary

This study introduces a high-throughput method combining phage display and high-density arrays to rapidly identify and characterize allergen clones from complex sources. This approach aids in understanding cross-reactivity and developing new allergy diagnostics and therapeutics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural aspects and clinical relevance of Aspergillus fumigatus antigens/allergens.

Medical mycology·2018
Same author

Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer.

Allergy·2018
Same author

Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice.

Allergy·2016
Same author

Artificial human sera: a breakthrough?

Allergy·2016
Same author

EAACI Molecular Allergology User's Guide.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2016
Same author

Global Allergy Forum and 3rd Davos Declaration 2015: Atopic dermatitis/Eczema: challenges and opportunities toward precision medicine.

Allergy·2016

Area of Science:

  • Molecular Biology
  • Immunology
  • Biotechnology

Background:

  • Complex allergenic sources contain numerous IgE-binding molecules.
  • Characterizing these molecules is crucial for mimicking whole allergen extracts and understanding allergic reactions.
  • Current methods for characterizing allergen repertoires are often slow and costly.

Purpose of the Study:

  • To develop a high-throughput, cost-effective technology for identifying and characterizing allergen clones.
  • To analyze whole allergenic repertoires from complex sources.
  • To provide a rational explanation for IgE-mediated cross-reactivity.

Main Methods:

  • Utilized phage display of cDNA libraries for selective enrichment of allergen-expressing clones using patient IgE.
  • Employed a high-throughput, quantitative technology for rapid identification of clones in enriched libraries.

Related Experiment Videos

  • Combined phage display with high-density arrays for comprehensive repertoire characterization.

    • Main Results:

    • Successfully identified and characterized diverse allergen clones from complex allergenic sources.
    • Discovered panels of related protein structures across different allergenic sources.
    • Identified secreted, cytoplasmic, and structural proteins, some with enzymatic activity.

    Conclusions:

    • The developed strategy enables fast and cost-effective characterization of whole allergenic repertoires.
    • This approach facilitates the discovery of novel allergens and related structures.
    • The findings offer a molecular basis for understanding IgE-mediated cross-reactivity in allergies.