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Related Experiment Videos

Thiols decrease cytokine levels and down-regulate the expression of CD30 on human allergen-specific T helper (Th) 0

A Bengtsson1, M Lundberg, J Avila-Cariño

  • 1Department of Medicine, Unit of Clinical Allergy Research, Karolinska Institutet, Stockholm, Sweden. asa.bengtsson@mb.ki.se

Clinical and Experimental Immunology
|April 12, 2001
PubMed
Summary
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N-acetyl-L-cysteine (NAC) and glutathione (GSH) reduce Th2 cytokines and CD30 expression, potentially favoring Th1 responses. NAC may be a therapeutic agent for Th2-mediated diseases like allergies.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • N-acetyl-L-cysteine (NAC), a glutathione (GSH) precursor, is used in various medical treatments.
  • GSH and NAC enhance T cell proliferation and IL-2 production in vitro.
  • CD30 is a surface antigen expressed on activated T helper cells, particularly Th2 cells.

Purpose of the Study:

  • To investigate the effects of GSH and NAC on cytokine profiles and CD30 expression in human allergen-specific T cell clones (TCC).

Main Methods:

  • Human allergen-specific T cell clones (TCC) were stimulated with anti-CD3 antibodies.
  • Different concentrations of GSH and NAC were added to the TCC cultures.
  • Cytokine levels (IL-4, IL-5, IFN-gamma) and CD30 expression (surface and soluble) were analyzed.

Main Results:

Related Experiment Videos

  • Both GSH and NAC dose-dependently downregulated IL-4, IL-5, and IFN-gamma in Th0 and Th2 clones, with a notable decrease in IL-4.
  • Surface and soluble CD30 expression were reduced by GSH and NAC.
  • CD28 and CD40 ligand expression remained unchanged after NAC treatment.

Conclusions:

  • GSH and NAC promote a Th1 response by preferentially downregulating IL-4.
  • CD30 expression appears to be dependent on IL-4 or modulated by NAC.
  • NAC shows potential as a future therapeutic agent for Th2-related diseases, such as allergies, due to its effects on CD30.