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Related Experiment Videos

Mapping action potentials and calcium transients simultaneously from the intact heart.

K R Laurita1, A Singal

  • 1The Heart and Vascular Research Center, MetroHealth Campus, Case Western Reserve University, Cleveland, Ohio 44109, USA. klaurita@metrohealth.org

American Journal of Physiology. Heart and Circulatory Physiology
|April 12, 2001
PubMed
Summary

This study developed an optical mapping system to simultaneously measure cardiac action potentials and calcium transients in guinea pig hearts. The system minimizes spectral overlap errors, enabling simultaneous optical mapping of these crucial electrophysiological signals.

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Area of Science:

  • Cardiovascular Physiology
  • Biomedical Optics
  • Electrophysiology

Background:

  • Intracellular calcium handling is critical for cardiac electrophysiology.
  • Simultaneous measurement of action potentials and calcium transients is essential for understanding heart function.
  • Previous methods faced limitations in simultaneously capturing these dynamic processes.

Purpose of the Study:

  • To develop and validate an optical mapping system for simultaneous measurement of action potentials and calcium transients in the intact guinea pig heart.
  • To assess and minimize spectral overlap errors between fluorescent indicators di-4-ANEPPS and indo 1.
  • To enable precise spatiotemporal analysis of cardiac electrophysiology.

Main Methods:

  • Utilized a dual-wavelength optical mapping system with guinea pig hearts.

Related Experiment Videos

  • Employed fluorescent indicators di-4-ANEPPS for action potentials and indo 1 for calcium transients.
  • Optimized excitation/emission filters (515±5 nm/>695 nm for action potentials; 365±25 nm/485±5 nm for calcium transients) to minimize spectral overlap.
  • Quantified spectral overlap error through in vitro measurements.

    • Main Results:

    • The developed system successfully measured action potentials and calcium transients simultaneously at 256 sites.
    • Spectral overlap error was minimal for action potential measurements (1.7±1.0%) and negligible for calcium transients (0%).
    • Simultaneous recordings showed negligible error due to fluorescence emission overlap, validated by isochrone maps.

    Conclusions:

    • The optical mapping system effectively overcomes spectral overlap challenges for simultaneous measurement of cardiac action potentials and calcium transients.
    • This technique provides a robust tool for investigating complex electrophysiological phenomena in the intact heart.
    • The validated method advances the capability for real-time, multi-parametric analysis of cardiac function.