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Evaluation of total PSA assay on vitros ECi and correlation with Kryptor-PSA assay
B Cassinat1, M Wacquet, M E Toubert
1Nuclear Medicine Department, Hôpital Saint Louis, 1 Av Claude Vellefaux, 75010 Paris, France.
Background:
An increasing number of multiparametric immuno-analysers for PSA assays are available. As different immuno-assays may vary in their analytical quality and their accuracy for the follow-up of patients, expertise is necessary for each new assay.
Methods:
The PSA assay on the Vitros-ECi analyser has been evaluated and compared with the PSA assay from the Kryptor analyser.
Results:
Variation coefficients were 0.91 to 1.98% for within-run assays, and 4.2% to 5.4% for interassay (PSA levels = 0.8 microgram/L to 33.6 micrograms/L). Dilution tests showed 93 to 136% recovery until 70 micrograms/L PSA. Functional sensitivity was estimated at 0.03 microgram/L. Equimolarity of the test was confirmed. Correlation of PSA levels measured with Vitros-ECi and Kryptor analysers displayed a correlation coefficient r2 of 0.9716. The half-lives and doubling times of PSA were similar using both methods.
Conclusion:
Vitros-ECi PSA assay meets the major criteria for the management of prostate cancer patients.