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Related Experiment Videos

Improved solid-phase extraction method for systematic toxicological analysis in biological fluids.

T Soriano1, C Jurado, M Menéndez

  • 1Instituto Nacional de Toxicología, Sevilla, Spain. quim@sev.inaltox.es

Journal of Analytical Toxicology
|April 13, 2001
PubMed
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This study presents a new method for simultaneously detecting and quantifying drugs of abuse and prescribed medications in biological samples. The validated procedure ensures reliable analysis with high recovery rates for various drug classes.

Area of Science:

  • Forensic Toxicology
  • Analytical Chemistry

Background:

  • Accurate detection of drugs in biological fluids is crucial for clinical and forensic toxicology.
  • Existing methods may lack the simultaneous capability for diverse drug classes or require complex procedures.

Purpose of the Study:

  • To develop and validate a simultaneous method for qualitative and quantitative determination of drugs of abuse and prescribed medications.
  • To analyze various biological matrices including blood, urine, bile, and gastric contents.

Main Methods:

  • Solid-phase extraction using Bond-Elut Certify columns.
  • Analysis via gas chromatography-nitrogen-phosphorus detection (GC-NPD) and confirmation using gas chromatography-mass spectrometry (GC-MS).
  • Sample pretreatment involved sonication and enzymatic hydrolysis; specific internal standards and pH-dependent extraction were employed.

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Main Results:

  • The developed method allows for simultaneous analysis of opiates, cocaine, amphetamines, tricyclic antidepressants, phenothiazines, and benzodiazepines.
  • Optimized extraction conditions ensured reliable systematic analysis.
  • Achieved recovery rates for the tested compounds consistently exceeded 61.02%.

Conclusions:

  • The validated method provides a robust and efficient approach for comprehensive drug screening in biological samples.
  • This technique is suitable for both qualitative and quantitative assessments, aiding in clinical diagnostics and forensic investigations.