Related Experiment Videos
A simple and rapid method for intra- and interspecific transformation of Bacillus subtilis on solid media by DNA in
1Department of Applied Microbial Technology, Sojo University, Kumamoto, Japan. akamatsu@bio.sojo-u.ac.jp
Bioscience, Biotechnology, and Biochemistry
|April 17, 2001
Abstract:
A simple method for intra- and interspecific transformation of Bacillus subtilis on solid media has been devised with DNA in protoplast lysates, 0.8% agar, glutamate, and yeast extract. The transformation frequency is 2.3 x 10(3) transformants per microg DNA, 10-20 times higher than that for conventional transformation on solid media. The method can be applicable to transformation in microtiter plates.