Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Downstream Processing

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of two wheat cultivars on physico-chemical properties of wheat flours and digesta from two broiler chicken lines (D+ and D-) differing in digestion capacity.

British poultry science·2007

Same author

Characterisation and foaming properties of hydrolysates derived from rapeseed isolate.

Colloids and surfaces. B, Biointerfaces·2006

Same author

Identification of IgE-binding epitopes on gliadins for patients with food allergy to wheat.

Allergy·2005

Same author

Rheological interfacial properties of plant protein-arabic gum coacervates at the oil-water interface.

Biomacromolecules·2005

Same author

Large scale purification of rapeseed proteins (Brassica napus L.).

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2005

Same author

Adsorption kinetics and rheological interfacial properties of plant proteins at the oil-water interface.

Biomacromolecules·2004

Same journal

High performance liquid chromatographic analysis of St. John's wort with photodiode array detection.

Journal of chromatography. B, Biomedical sciences and applications·2002

Same journal

Novel and simple two-step purification of a full-length rat glucocorticoid-receptor expressed in a baculovirus system.

Journal of chromatography. B, Biomedical sciences and applications·2002

Same journal

Stereoselective analysis of fluvastatin in human plasma for pharmacokinetic studies.

Journal of chromatography. B, Biomedical sciences and applications·2002

Same journal

Metabolism of methandrostenolone in the horse: a gas chromatographic-mass spectrometric investigation of phase I and phase II metabolism.

Journal of chromatography. B, Biomedical sciences and applications·2002

Same journal

Quantitative analysis of fentanyl in rat plasma by gas chromatography with nitrogen-phosphorus detection.

Journal of chromatography. B, Biomedical sciences and applications·2002

Same journal

Liquid chromatography-mass spectrometry assay of a thiadiazole derivative in mice: application to pharmacokinetic studies.

Journal of chromatography. B, Biomedical sciences and applications·2002

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Published on: October 4, 2012

Ultrafiltration to fractionate wheat polypeptides.

S Berot¹, Y Popineau, J P Compoint

¹Unité de Biochimie et Technologie des Protéines, INRA, Nantes, France.

Journal of Chromatography. B, Biomedical Sciences and Applications

|April 17, 2001

Summary

This summary is machine-generated.

Ultrafiltration effectively separated wheat gliadin polypeptides based on hydrophobicity. Hydrophobic fractions enhanced foaming properties, while hydrophilic fractions did not, offering a novel method for functional ingredient development.

More Related Videos

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

Published on: April 13, 2018

Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

Published on: February 18, 2022

Related Experiment Videos

Last Updated: Jun 25, 2026

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Published on: October 4, 2012

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

Published on: April 13, 2018

Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

Published on: February 18, 2022

Area of Science:

Food Science
Biochemistry
Separation Science

Background:

Wheat gliadins are complex proteins yielding various polypeptides upon hydrolysis.
Fractionating these polypeptides is crucial for understanding and utilizing their functional properties, such as foaming.

Purpose of the Study:

To investigate the efficacy of ultrafiltration for fractionating wheat gliadin hydrolysates.
To characterize the separated polypeptide fractions based on their physicochemical properties and foaming capabilities.

Main Methods:

Limited chymotryptic hydrolysis of wheat gliadins.
Ultrafiltration using inorganic ZrO2-based membranes at acidic pH.
Characterization of permeate and retentate fractions using chromatography and foaming assays.

Main Results:

Ultrafiltration successfully separated polypeptides into hydrophilic (permeate) and hydrophobic (retentate) fractions.
Permeate fractions were rich in hydrophilic, poorly charged polypeptides (84-90%).
Retentate fractions contained 75-88% hydrophobic polypeptides, exhibiting significantly enhanced foaming properties compared to whole hydrolysates.

Conclusions:

Ultrafiltration provides an effective method for fractionating wheat gliadin hydrolysates based on polypeptide hydrophobicity.
The hydrophobic polypeptide-rich retentate fractions demonstrate superior foaming capabilities, suggesting potential applications in food formulations.