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Related Experiment Videos

Reversible shear-mediated platelet dysfunction during cardiac surgery as assessed by the PFA-100 platelet function

T F Slaughter1, G Sreeram, A D Sharma

  • 1Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina 27710, USA. slaug003@mc.duke.edu

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
|April 17, 2001
PubMed
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Cardiac surgery impairs platelet function, affecting bleeding risk. The PFA-100 test shows potential for identifying patients unlikely to benefit from platelet transfusions post-surgery.

Area of Science:

  • Cardiovascular Surgery
  • Hematology
  • Platelet Physiology

Background:

  • Cardiac surgery involves cardiopulmonary bypass (CPB), which can affect platelet function.
  • Assessing shear-mediated platelet dysfunction is crucial for managing post-operative bleeding.

Purpose of the Study:

  • To evaluate changes in shear-mediated platelet function during cardiac surgery.
  • To determine if the PFA-100 assay can predict post-operative bleeding.

Main Methods:

  • Platelet aggregation and PFA-100 closure times were measured in patients during cardiac surgery.
  • Associations between PFA-100 closure times and post-operative bleeding were analyzed.

Main Results:

  • Collagen/epinephrine closure times were prolonged throughout surgery.

Related Experiment Videos

  • Collagen/adenosine-5'-diphosphate (ADP) closure times significantly increased with heparin and CPB, returning to baseline after protamine.
  • Platelet aggregation in response to ADP remained impaired post-CPB in most patients.

    • Conclusions:

    • Both intrinsic and extrinsic factors contribute to reversible platelet dysfunction during CPB.
    • The PFA-100 assay may help identify patients who will not benefit from platelet transfusions after CPB.