Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mammalian prohibitin proteins respond to mitochondrial stress and decrease during cellular senescence.

P J Coates1, R Nenutil, A McGregor

  • 1Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, United Kingdom. p.j.coates@dundee.ac.uk

Experimental Cell Research
|April 17, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biopsychosocial factors and low back pain in military personnel with lower limb loss: the ADVANCE study.

BMJ military health·2025
Same author

From the clinic to the wards, the evolution of penicillin allergy testing by non-allergists in a UK hospital.

The Journal of hospital infection·2024
Same author

Diabetes mellitus is not a predictor of poor TB treatment outcomes.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2023
Same author

A laboratory Brucella exposure in a UK hospital: a Swiss cheese model?

The Journal of hospital infection·2019
Same author

Intrinsic proteotoxic stress levels vary and act as a predictive marker for sensitivity of cancer cells to Hsp90 inhibition.

PloS one·2018
Same author

Cognitive effects of a 30-min aerobic exercise bout on adults with overweight/obesity and type 2 diabetes.

Obesity science & practice·2017
Same journal

The AP2α/PDHA1 signaling pathway promotes lung cancer progression by mediating aerobic glycolysis and inhibiting cuproptosis.

Experimental cell research·2026
Same journal

Distributed transcription activity in DLX proteins defies conventional mapping of a transcription activation domain.

Experimental cell research·2026
Same journal

Extracellular release of cytotoxic aggregates from HSV-1-replicating SH-SY5Y cells: involvement of alpha-synuclein and poly-ubiquitin conjugates.

Experimental cell research·2026
Same journal

MiR-1281 downregulates LMX1B to inhibit gastric cancer development.

Experimental cell research·2026
Same journal

Corrigendum to "Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells" [Exp. Cell Res., 326, 1, 1 August 2014, Pages 78-89].

Experimental cell research·2026
Same journal

Neutrophilic differentiation of promyelocytic HL-60 cells is associated with increased expression of terminal galactose residues on the cell surface.

Experimental cell research·2026
See all related articles

Mammalian prohibitins (Phb1p and Phb2p) reside in the inner mitochondrial membrane and are crucial for mitochondrial metabolism and aging. Their expression correlates with metabolic stress and tumor development, not cell proliferation or apoptosis.

Area of Science:

  • Mitochondrial biology
  • Cellular aging
  • Oncoprotein interactions

Background:

  • Prohibitins (Phb1p and Phb2p) are implicated in diverse cellular functions, including cell cycle, apoptosis, mitochondrial enzyme assembly, and aging.
  • Previous research suggested varied roles, necessitating a clearer understanding of their localization and specific functions in mammalian cells.

Purpose of the Study:

  • To elucidate the precise localization and functional significance of mammalian prohibitins (Phb1p and Phb2p).
  • To investigate the correlation between prohibitin expression and cellular processes like metabolism, proliferation, apoptosis, aging, and oncogenesis.

Main Methods:

  • Immunofluorescence and biochemical assays to determine prohibitin localization within mitochondria.
  • Analysis of prohibitin co-expression patterns during development and in adult tissues.

Related Experiment Videos

  • Examination of prohibitin expression levels in response to various cellular stresses (metabolic, oxidative, heat shock) and in primary human tumors and senescent fibroblasts.
  • Investigation of the role of Myc oncoprotein in regulating prohibitin gene expression via promoter analysis.

    • Main Results:

    • Mammalian prohibitins (Phb1p and Phb2p) are localized to the inner mitochondrial membrane and exist as a stable complex.
    • Expression levels correlate with mitochondrial metabolism and aging, but not directly with cellular proliferation or apoptosis.
    • Prohibitins are upregulated in human tumors and induced by metabolic stress, with Myc oncoprotein shown to regulate their expression.

    Conclusions:

    • Mammalian prohibitins are integral components of the inner mitochondrial membrane, playing a conserved role in regulating mitochondrial respiratory activity.
    • Their expression is linked to aging and metabolic homeostasis, and their dysregulation is observed in cancer.
    • The findings support a critical role for prohibitins in mitochondrial function and the aging process.