Preparation of oligodeoxynucleotides containing 5-(N-methylpiperazinyl) and 5-benzyloxymethyl uracils
A E Abdel Megied1, O M Ali, T Kofoed
1Department of Chemistry, Faculty of Science, Menoufia University, Shebien El-Koam, Egypt. abdel_megied@yahoo.com
Nucleosides, Nucleotides & Nucleic Acids
|April 17, 2001
Abstract:
Deprotected compounds 1 and 9 were allowed to react with 4,4'-dimethoxytrityl chloride in pyridine to give 5'-O-DMT nucleosides 2 and 10. The 3'-phosphoramidites 4 and 11 were incorporated into oligodeoxynucleosides (ODNs). The hybridization properties of the modified ODNs with their complementary DNA strands were studied. Interesting results were obtained when 11 was inserted as a bulged nucleoside into TWAs, duplexes, and triplexes.
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