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Published on: October 12, 2017
Measurement of copper-binding sites on low density lipoprotein
A Roland1, R A Patterson, D S Leake
1Cell and Molecular Biology Research Division, School of Animal and Microbial Sciences, The University of Reading, Reading, Berkshire, UK. a.roland@reading.ac.uk
Arteriosclerosis, Thrombosis, and Vascular Biology
|April 17, 2001
Summary
This study quantifies copper binding sites on low-density lipoprotein (LDL), finding approximately 39 sites per LDL particle. Certain flavonoids effectively inhibit copper binding to LDL, suggesting antioxidant potential.
Area of Science:
- Biochemistry
- Cardiovascular Research
- Analytical Chemistry
Background:
- Copper-mediated oxidation of low-density lipoprotein (LDL) is implicated in atherosclerosis.
- The binding of copper to LDL is a presumed prerequisite for its oxidation, but binding estimates vary.
- Understanding copper-LDL interactions is crucial for developing targeted therapies.
Purpose of the Study:
- To develop and validate a method for quantifying copper binding to LDL.
- To investigate the effect of LDL oxidation and chemical modifications on copper binding capacity.
- To assess the impact of specific flavonoids on copper binding to LDL.
Main Methods:
- Development and validation of an equilibrium dialysis assay in a MOPS-buffered system.
- Quantification of copper binding sites on native and oxidized LDL.
- Assessment of copper binding after chemical modification of apolipoprotein B-100 residues.
- Evaluation of flavonoid effects on copper binding to LDL.
Main Results:
- Identified an average of 38.6 copper binding sites per LDL particle.
- Copper binding capacity increased with LDL oxidation.
- Histidyl and lysyl residue modification significantly reduced copper binding sites.
- Myricetin, quercetin, and catechin significantly decreased copper binding to LDL.
Conclusions:
- The developed assay accurately quantifies copper binding sites on LDL.
- Copper binding to LDL is influenced by LDL oxidation state and protein modifications.
- Specific flavonoids demonstrate potential in inhibiting copper binding to LDL, offering therapeutic avenues.
