Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Room temperature activates human blood platelets.

E Maurer-Spurej1, G Pfeiler, N Maurer

  • 1Canadian Blood Services (EM-S, DVD), Vancouver. emaurer@interchange.ubc.ca

Laboratory Investigation; a Journal of Technical Methods and Pathology
|April 17, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outcomes in stage IIA versus stage IIB/III in the PALLAS trial [ABCSG-42/AFT-05/PrE0109/BIG-14-13]).

Breast cancer research : BCR·2025
Same author

Drug-drug interactions between palbociclib and proton pump inhibitors in early breast cancer: an exploratory analysis of PALLAS (ABCSG-42/AFT-05/BIG-14-13/PrE0109).

ESMO open·2025
Same author

Correction: Informal carers' experiences in everyday life and the use of digital assistive technology for time management in persons with dementia or mild cognitive impairment.

BMC geriatrics·2024
Same author

Informal carers' experiences in everyday life and the use of digital assistive technology for time management in persons with dementia or mild cognitive impairment.

BMC geriatrics·2024
Same author

Effect of concomitant statin treatment in postmenopausal patients with hormone receptor-positive early-stage breast cancer receiving adjuvant denosumab or placebo: a post hoc analysis of ABCSG-18.

ESMO open·2022
Same author

Raman spectroscopy of stored red blood cell concentrate within sealed transfusion blood bags.

The Analyst·2018
Same journal

Changes in Three-Dimensional Intrahepatic Biliary Structures in Patients With Hepatobiliary Diseases Visualized Using Tissue-Clearing Methods.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Genome-wide SNP-based Profiling of Loss of Heterozygosity Reveals Distinct Molecular Subgroup-specific Patterns in Gastrointestinal Stromal Tumors (GIST).

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

AI-Assisted HER2 Scoring in Breast Cancer: Diagnostic Agreement and Understanding Discordance.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Corrigendum to "POU2F3 in Small Cell Lung Cancer (SCLC): Diagnostic Utility in Neuroendocrine-Low/Negative SCLC and Discrimination From Other Thoracic Malignancies and Other Small Blue Round Cell Tumors" [Laboratory Investigation 2026;106(6):106124].

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Assessing the Effects of a 3D Pathology Tissue-Processing Workflow on Downstream Molecular Analyses.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Transcription Factor Ets-1 Is a Central Regulator of Redox Balance and Liver Regeneration Through epidermal growth factor (EGF) and transforming growth factor-β (TGF-β) 1 Signaling.

Laboratory investigation; a journal of technical methods and pathology·2026
See all related articles

Room temperature (20°C) significantly activates human platelets, altering their morphology and increasing serotonin content. This finding challenges the assumption that cool temperatures do not affect platelet function, impacting transfusion storage and hypothermia-related bleeding risks.

Area of Science:

  • Hematology
  • Biophysics
  • Cell Biology

Background:

  • Clinical observations suggest hypothermia impairs hemostasis, yet the effect of temperatures above 20°C on platelet function is poorly understood.
  • Existing assumptions state that temperatures from 20°C to 42°C do not activate platelets, a notion contradicted by clinical hemostatic failure in hypothermia.

Purpose of the Study:

  • To investigate the impact of temperatures between 20°C and 42°C on human platelet activation.
  • To determine if room temperature (20°C) activates platelets and affects their serotonin content.

Main Methods:

  • Platelet morphology was assessed using light microscopy, dynamic and static light scattering, and electron microscopy.
  • Platelet activation was quantified by measuring activated glycoprotein (GP) IIb-IIIa expression via flow cytometry.

Related Experiment Videos

  • Platelet serotonin content was analyzed in the presence of serotonin re-uptake inhibitors.

    • Main Results:

    • Exposure to 20°C for 10 minutes induced significant morphological changes in platelets compared to resting platelets at 37°C.
    • 25-30% of platelets expressed activated GPIIb-IIIa after 10 minutes at 20°C.
    • Platelets at 20°C showed doubled serotonin content compared to resting platelets, while 42°C induced no activation markers.

    Conclusions:

    • Room temperature (20°C) is a significant platelet activator, affecting morphology and serotonin levels.
    • These findings may explain platelet dysfunction during cold storage for transfusion and hemostatic issues post-hypothermia.