Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Identification of Ras-regulated genes by representational difference analysis.

J M Shields1, C J Der, S Powers

  • 1Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-7295, USA.

Methods in Enzymology
|April 18, 2001
PubMed
Summary

RNA arbitrarily (RDA) profiling offers a quick, simple, and affordable method for identifying genes altered by Ras transformation. This research may reveal new anticancer drug targets and mechanisms of cell transformation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigating the role of executive function in brain responses to infant crying among new parents.

Social cognitive and affective neuroscience·2026
Same author

Natural History of Common Autologous Arteriovenous Fistulae: Consequences for Planning of Dialysis.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2016
Same author

The influences of chronic illness and ego development on self-esteem in diabetic and psychiatric adolescent patients.

Journal of youth and adolescence·2013
Same author

An approach to evaluating adolescent adaptive processes: Validity of an interview-based measure.

Journal of youth and adolescence·2013
Same author

Sex differences within the family: Studies of adolescent and parent family interactions.

Journal of youth and adolescence·2013
Same author

Rho guanine nucleotide exchange factors: regulators of Rho GTPase activity in development and disease.

Oncogene·2013

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Ras proteins are key regulators of cell signaling pathways.
  • Aberrant Ras signaling is implicated in numerous human cancers.
  • Understanding gene expression changes during Ras transformation is crucial for cancer therapy.

Purpose of the Study:

  • To present RNA arbitrarily (RDA) profiling as an efficient method for identifying differentially expressed genes.
  • To characterize genes affected by Ras transformation.
  • To explore potential therapeutic targets for anticancer drug development.

Main Methods:

  • RNA arbitrarily (RDA) profiling was employed to compare gene expression profiles.
  • Specific genes were identified and characterized based on their altered expression levels.

Related Experiment Videos

Main Results:

  • RDA profiling demonstrated effectiveness in identifying genes with aberrant expression due to Ras transformation.
  • The method is technically straightforward and cost-effective.

Conclusions:

  • RDA is a valuable tool for studying gene expression changes in Ras-transformed cells.
  • Identifying these genes offers insights into transformation mechanisms and potential drug targets for cancer treatment.