Isolation of effector-selective Ras mutants by yeast two-hybrid screening

Ras mutants displaying selective target interactions will often display partial loss of function phenotypes when expressed in cells. Thus the isolation of mutations in Ras and Ras family members has proved to be a productive approach for testing the requirement of specific target interactions to mediate downstream responses. The procedures outlined here greatly simplify the isolation of such mutants, and it is hoped will contribute to a better understanding of Ras effector function.