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Updated: Aug 6, 2026

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
Published on: October 19, 2013
Isolation and characterization of posteriorly restricted genes in the zebrafish gastrula
C G Sagerström1, B A Kao, M E Lane
1Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts, USA.
Abstract:
In order to understand anteroposterior axis formation in vertebrates, we have used subtractive hybridization to clone genes expressed posteriorly in the zebrafish gastrula-stage embryo. Here we report the initial characterization of eight clones isolated from this screen. We find that all eight genes are expressed in posteriorly restricted domains, suggesting that they are involved in regulating posterior development during zebrafish embryogenesis.

