Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diabetes and mass spectrometry.

A Lapolla1, D Fedele, P Traldi

  • 1Dipartimento di Scienze Mediche e Chirurgiche, Cattedra di Malattie del Metabolismo - Padova University, Via Vendramini 7, 35100 Padova, Italy.

Diabetes/Metabolism Research and Reviews
|April 18, 2001
PubMed
Summary

Mass spectrometry (MS) effectively monitors diabetes by quantifying protein glycation. This technique analyzes hemoglobin and immunoglobulin glycation, providing insights into metabolic control and oxidative stress in diabetic patients.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comments on TOBOGM study: treatment of gestational diabetes mellitus diagnosed early in pregnancy.

Journal of endocrinological investigation·2024
Same author

Gestational diabetes mellitus in patients undergoing assisted reproductive techniques or conceiving spontaneously: an analysis on maternal and foetal outcomes.

Journal of endocrinological investigation·2024
Same author

Short-term effectiveness of dapagliflozin versus DPP-4 inhibitors in elderly patients with type 2 diabetes: a multicentre retrospective study.

Journal of endocrinological investigation·2023
Same author

Maintenance of clinical response after short treatment with cemiplimab for advanced squamous cell carcinoma.

Journal of the European Academy of Dermatology and Venereology : JEADV·2022
Same author

The unexplored role of sedentary time and physical activity in glucose and lipid metabolism-related placental mRNAs in pregnant women who are obese: the DALI lifestyle randomised controlled trial.

BJOG : an international journal of obstetrics and gynaecology·2021
Same author

Comment: Characteristics and outcomes of pregnant women with type 1 or type 2 diabetes-a 5-year national population-based cohort study.

Journal of endocrinological investigation·2021

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Clinical Chemistry

Background:

  • Non-enzymatic protein glycation is a key process in diabetes mellitus.
  • Traditional methods for assessing glycation have limitations in sensitivity and specificity.
  • Mass spectrometry (MS) offers a powerful tool for investigating protein glycation.

Purpose of the Study:

  • To evaluate the utility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) for assessing protein glycation in diabetes.
  • To investigate glycation patterns in hemoglobin and immunoglobulins (IgG) in healthy and diabetic subjects.
  • To correlate glycation levels with metabolic control and oxidative stress in diabetes.

Main Methods:

  • Utilized matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) to determine the molecular weight of intact proteins.

Related Experiment Videos

  • Applied MALDI/MS to analyze plasma proteins, including hemoglobin and IgG, from healthy and diabetic individuals.
  • Quantified the number of glucose molecules condensed on proteins and identified glyco-oxidized species.

    • Main Results:

    • MALDI/MS accurately quantified protein glycation, enabling precise monitoring of metabolic control in diabetic patients.
    • Analysis of hemoglobin revealed similar glycation levels in alpha- and beta-globins, with co-occurrence of glyco-oxidized species indicating oxidative stress.
    • For IgG, MALDI/MS determined total glycation and identified the fragment antigen-binding (Fab) moiety as the most glycated region.

    Conclusions:

    • MALDI/MS is a valuable technique for precise monitoring of diabetes through the assessment of protein glycation.
    • Protein glycation patterns in hemoglobin provide insights into metabolic control and oxidative stress.
    • The glycation of the Fab moiety of IgG may contribute to immunological impairments observed in diabetes, potentially affecting antibody-antigen recognition.