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Updated: Aug 8, 2026

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
Published on: April 6, 2012
Fc(epsilon)RI as a paradigm for a lipid raft-dependent receptor in hematopoietic cells
1Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University, Ithaca, NY 14853-1301, USA.
Abstract:
Lipid domains or rafts are currently embraced by immunologists as critical participants in receptor-mediated signaling events occurring at the plasma membrane. This view of membrane heterogeneity and its functional importance is supported by many years of different experimental approaches. We can now refine our investigations, moving beyond the simple models to ask more detailed questions about structural properties and mechanistic interactions. As highlighted for the IgE receptor (Fc(epsilon)RI), new information about initial engagement with src family kinases, cytoskeletal regulation, and coupling with downstream signaling is beginning to emerge.
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