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Related Experiment Videos

Probing confined fields with single molecules and vice versa.

B Sick1, B Hecht, U P Wild

  • 1Physical Chemistry Laboratory, Swiss Federal Institute of Technology, ETH-Zentrum, CH-8092 Zürich, Switzerland.

Journal of Microscopy
|April 20, 2001
PubMed
Summary
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Single dye molecules precisely map electric field distributions in a high numerical aperture lens focus. This technique verifies simulations and enables determination of molecular dipole orientation, crucial for advanced microscopy.

Area of Science:

  • Optics and Photonics
  • Molecular Biophysics
  • Nanotechnology

Background:

  • Understanding light field distribution is crucial for nanoscale imaging and manipulation.
  • High numerical aperture (NA) lenses concentrate light, creating complex electric field patterns.
  • Dye molecules can act as local probes for optical field characterization.

Purpose of the Study:

  • To spatially map the squared electric field components in the focus of a high NA lens using single dye molecules.
  • To quantitatively verify simulated field distributions with experimental data.
  • To demonstrate engineering of electric field components using annular illumination.

Main Methods:

  • Utilizing single dye molecules as local probes.
  • Employing fluorescence excitation mapping to record experimental data.

Related Experiment Videos

  • Comparing experimental maps with simulated electric field distributions.
  • Applying annular illumination to control field polarization.

    • Main Results:

    • Accurate mapping of squared electric field components achieved.
    • Simulated and experimental field distributions show quantitative agreement.
    • Annular illumination successfully engineered field distribution at a dielectric/air interface.
    • Electric field components in all directions acquired comparable magnitudes.
    • Determined 3D orientation of molecular absorption dipoles.

    Conclusions:

    • Single dye molecules are effective probes for mapping optical electric fields.
    • Annular illumination offers control over focal field characteristics.
    • The method allows for determination of molecular dipole orientation.
    • Findings are relevant for tip-enhanced near-field optical microscopy and other applications requiring precise light field control.