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A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

Immunophenotyping.

J J Bleesing¹, T A Fleisher

¹Department of Laboratory Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892-1508, USA.

Seminars in Hematology

|April 20, 2001

Summary

This summary is machine-generated.

Multiparameter flow cytometry immunophenotyping aids in diagnosing nonmalignant conditions by counting lymphocytes and identifying cell markers. This technique is crucial for human immunodeficiency virus (HIV) monitoring and immune system disorder assessment.

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Area of Science:

Clinical immunology
Flow cytometry applications
Cellular analysis

Background:

Immunophenotyping uses multiparameter flow cytometry to analyze leukocytes in nonmalignant conditions.
It involves counting lymphocyte populations and evaluating cell surface markers.

Purpose of the Study:

Focuses on clinical applications of immunophenotyping.
Highlights its role in CD4 T-cell counts and activation markers for HIV infection.
Addresses characterization of primary immunodeficiency and immune-mediated diseases.
Examines immune reconstitution after stem cell transplantation (SCT).

Main Methods:

Utilizes multiparameter flow cytometry.
Employs techniques for enumerating specific lymphocyte populations.
Evaluates cell surface markers for diagnostic purposes.

Main Results:

Demonstrates the utility of immunophenotyping in various nonmalignant hematologic and immunologic conditions.
Shows advancements in flow cytometry, including quantitative methods and single-platform technology.
Contributes to a deeper understanding of immunologic disorders.

Conclusions:

Immunophenotyping is a powerful tool for diagnosing and monitoring nonmalignant conditions.
Its applications are vital in managing HIV, primary immunodeficiencies, and immune reconstitution post-SCT.
Ongoing developments in flow cytometry enhance its clinical utility.